Staining of bone marrow aspiration slides: novel immediate staining Vs EDTA–stored samples

I. Histochemical Staining of Bone Marrow Smears

Histochemical procedures for PMN granule enzymes were carried out on smears prepared from normal rabbit bone marrow, and the smears were examined by light microscopy. For each of the enzymes tested, azo dye and heavy metal techniques were utilized when possible. The distribution and intensity of each reaction were compared to the distribution of azurophil and specific granules in developing PMN...

The Role of Bone Marrow Aspiration and Bone Marrow Biopsy in Diagnosis of Bone Marrow Metastases

Background: Bone marrow is the site of many malignant disorders and it is one of the common places for solid tumors to metastasize. Examination of the bone marrow aspirate and biopsy is a routine procedure performed for assessment of various conditions such as cytopenias, hematologic neoplasms, nonmalignant disorders and metastatic neoplasms. Methods: The patients were referred to the Hema...

Staining Reactions with Slides Treated with CsCl

Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related sta...

Quality Improvement Study: Bone Marrow (BM) Biopsy H&E Staining

A Quality Assurance program is an integral part of any anatomic pathology laboratory. As in many industries, processes are established to ensure that high levels of practice standards are met in order to reduce errors and improve patient safety. To be successful, a program should be customized to meet the needs of the individual organization. An effective quality assessment evaluation must enco...

An automatic staining machine for blood and marrow films on slides.